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Record identifier : 567827
Personal Name - Primary Intelectual Responsibility : Fortin, Doris Laurence
Title and statement of responsibility : Characterization of D108 Ner and its Escherichia coli homolog Nlp [Thesis]
Publication, Distribution,Etc. : McGill University (Canada),, 1998
Language of the Item : eng
Dissertation of thesis details and type of degree : M.Sc.
Body granting the degree : , McGill University (Canada),
Summary or Abstract : Mu and D108 are temperate bacteriophages which can follow two possible modes of growth after infection of Escherichia coli . The bacteriophages can follow the lytic cycle, in which the bacteriophage genome is replicated and progeny phages are produced, or the lysogenic cycle, in which the bacteriophage remains dormant in the E. coli genome. In this case, the bacteriophage's DNA is replicated along with the host chromosome. Central to the lytic/lysogenic switch, is the Ner protein. Ner is a small, basic, DNA-binding protein. To better understand the mechanism by which bacteriophage D108 regulates its lytic/lysogenic switch, a plasmid containing the D108 left end was mutagenized by hydroxylamine treatment or by transformation into a mutator strain of E. coli , in order to isolate Ner proteins which would be deficient in repressor activity..
Topical Name Used as Subject : Molecular biology
Information of biblio record : TL
 
 
 
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